报告题目：Quantitative modeling of microRNA-mediated regulation on competing endogenous RNAs
报告人: 汪小我，清华大学自动化系、清华信息科学与技术国家实验室副教授
主持人：蔡军 研究员
时 间：2015年4月13日（星期一）下午2:00-3:00
所在: 基因组所 5层集会室
报告人简介：汪小我，划分于2003年和2008年在清华大学自动化系获得工学学士学位和工学博士学位，现任清华大学自动化系、清华信息科学与技术国家实验室副教授。主要研究偏向为microRNA调控、高通量测序数据剖析要领、细胞衰老机制的系统生物学研究等。曾获全国优秀博士论文奖，并获得教育部新世纪优秀人才支持计划和国家自然科学基金优秀青年基金支持。
报告内容简介：Competing endogenous RNAs (ceRNAs) cross-regulate each other at post-transcriptional level by titrating shared microRNAs (miRNAs). We established a minimum ceRNA model to quantitatively analyze the behavior of the ceRNA regulation and implemented multi-fluorescent synthetic gene circuits in cultured human cells to validate our predictions. Our results suggested that the ceRNA effect is affected by the abundance of miRNA and ceRNAs, the number and affinity of binding site, and the mRNA degradation pathway determined by the degree of miRNA-mRNA complementarity. Furthermore, we found that a non-reciprocal competing effect between partial and perfect complementary targets is mainly due to different miRNA loss rate in these two types of repressions, which sheds light on utilizing such a competing model for rational design of effective siRNA.
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